Use of DNA microsatellites in beluga whale (Delphinapterus leucas) population genetics

  • 17 Pages
  • 4.83 MB
  • 1054 Downloads
  • English

Central and Arctic Region, Dept. of Fisheries and Oceans , Winnipeg, Man
White whale -- Genetics., DNA fingerprin
Statementby L.D. Maiers ... [et al.]
SeriesCanadian technical report of fisheries and aquatic sciences -- 2115, Canadian technical report of fisheries and aquatic sciences -- 2115.
ContributionsMaiers, L. D., Canada. Dept. of Fisheries and Oceans. Central and Arctic Region.
Classifications
LC ClassificationsSH"223"C35"no.2115
The Physical Object
Paginationv, 17 p. :
ID Numbers
Open LibraryOL20645933M

Get this from a library. Use of DNA microsatellites in beluga whale (Delphinapterus leucas) population genetics. [L D Maiers; Canada. Department of Fisheries and Oceans. Central and Arctic Region.]. Beluga whales don’t select their friends according to what Darwinists would expect, a new Florida Atlantic University study shows.1,2 The research findings are taken from ten Arctic beluga whale ranges, including Alaska’s Yakutat Bay, Cook Inlet, Norton Sound, Canada’s Husky Lakes, Russia’s Gulf of Anadyr, and a small population by Norway’s Svalbard.2 The study, led by Florida.

It has been postulated that beluga whale group structure centres around females with their calves of different a21 and is similar to the group structure in killer whales (Orcinus orca) and Author: Greg O’Corry-Crowe, Robert Suydam, Lori Quakenbush, Thomas G. Smith, Christian Lydersen, Kit M.

Kova. Beluga whales (Delphinapterus leucas) in North American waters migrate seasonally between wintering areas in broken pack ice and summering locations in estuaries and other open water areas in the Arctic and sub‐s from our previous investigation of beluga whale mitochondrial DNA (mtDNA) revealed genetic heterogeneity among beluga from different summering locations that was Cited by: Population structure of North American beluga whales (Delphinapterus leucas) based on nuclear DNA microsatellite variation and contrasted with the population structure revealed by mitochondrial DNA variation Delphinapterus leucas) based on nuclear DNA microsatellite variation and contrasted with the population structure revealed by mitochondrial.

(a) Mediannjoining network of haplotypes of the mtDNA control region ( bp), noted for beluga whales of the Russian Far East (our data) and waters off the northwestern coast of North America [23].

beluga whale, while all other 11 loci were from a humpback whale. Primer sequences and PCR cycling profiles followed those of the original authors. PCR amplifications followed the manufacturer’s instructions for the use of Ex Taq DNA polymerase (Takara Shuzo).

Amplified products were. Population structure in many Arctic marine mammal species reflects a dynamic interplay between physical isolating mechanisms and the extent to which dispersal opportunities are met.

We examined variation within mtDNA and eight microsatellite markers to investigate population structure and demographic history in beluga whales in the North Atlantic.

Genetic heterogeneity was observed. Estimates of genetic diversity and probabilities of identity for eight microsatellite loci in beluga whales. Values are given for the major beluga whale concentration areas in the western Nearctic. The level of microsatellite genetic diversity of the sperm whales used in this study was similar to that of other oceanic areas.

Lyrholm et al. () analyzed sperm whales from the North Atlantic, North Pacific, and Southern Hemisphere using nine sets of microsatellite primers, three (EV1, EV5, GATA28) of which were same as those in this study. Fifteen microsatellites were isolated from a beluga whale Delphinapterus leucas, genomic library.

The microsatellites were amplified in beluga obtained from two widely separated locations. An average of alleles per locus were detected and the average heterozygosity was with a range of – All microsatellites were polymorphic and 13 of the genotype distributions observed.

Macrogeographic patterns of population differentiation in beluga whales in the north Pacific Ocean: (A) within mitochondrial DNA and (B) across eight microsatellite loci.

Values for the frequency-based statistic, F st, are below the diagonal, values for the distance-based statistic, Φ st (mtDNA) and R st (nDNA), are above the diagonal.

Download Use of DNA microsatellites in beluga whale (Delphinapterus leucas) population genetics EPUB

Full Text; PDF ( K) PDF-Plus ( K) Citing articles; Population genetics and social organization of the sperm whale (Physeter macrocephalus) in the Azores inferred by microsatellite analysesA.

Pinela, a S. Quérouil, a S. Magalhães, a M. Silva, a R. Prieto, a J. Matos, b R. Santos a a Centro do IMAR da Universidade dos Açores, Departamento de Oceanografia e Pescas. Analysis of allelic distributions of microsatellite loci has shown that beluga whales that form the Sakhalin-Amur summer aggregation and the Shantar Islands summer ag-gregation belong to the same population (ӞӦӺөӭӡӟ ӧ ӣӯ., this book).

Of even greater interest is the issue whether be. The genetic structure of four summer aggregations of the Beluga Whale, Delphinapterus leucas, in Sakhalin Bay and Udskaya Bay, off the western coast of Kamchatka in the Sea of Okhotsk and in the Anadyr Estuary of the Bering Sea was analyzed through nucleotide sequencing of the mtDNA control region and detection of the allelic composition of nine microsatellite loci in nuclear DNA.

Tissue samples from beluga whales were collected over a year period from subsistence harvests and from live whales via biopsy.

Total DNA was extracted from each sample and screened for variation within bp of the mitochondrial genome and for polymorphism within eight hypervariable microsatellite markers, according to previously.

Details Use of DNA microsatellites in beluga whale (Delphinapterus leucas) population genetics EPUB

Genetic methods. Genomic DNA was extracted using the DNeasy® Blood and Tissue Kit (Qiagen, Chatsworth, CA, USA). To characterize the neutral polymorphism in this blue whale feeding ground, seven microsatellite loci previously described were used using PCR conditions as described previously (Buchanan et al.

; Valsecchi and Amos ; Palsbøll et al. ; Bérubé et al. It might seem like a “whale of tale,” but groundbreaking research from Florida Atlantic University’s Harbor Branch Oceanographic Institute is the first to demonstrate that just like human societies, beluga whales appear to value culture as well as their ancestral roots and family ties.

Through a detailed genetic study of kinship published in PLOS One, an international team of. 12 hours ago  Beluga whales Little Grey and Little White, have been freed from captivity in China to swim in the sea for the first time in nearly a decade.

The Sea Life Trust took the whales to. Use of DNA microsatellites in beluga whale (Deiphinapterus leucas) population genetics. Canadian Technical Report of Fisheries and Aquatic Sciences.

A groundbreaking study is the first to analyze the relationship between group behaviors, group type, group dynamics, and kinship of beluga whales in 10 locations across the Arctic. Results show.

Phylogenetic analysis of the deduced amino acid sequences from the DNA polymerase gene fragments of all 10 beluga whale virus isolates, using homologous sequences from selected members of all three subfamilies of the Herpesviridae family, showed that the beluga whale isolates shared identities of %–% among themselves, with the highest.

The beluga whale (/ b ɪ ˈ l uː ɡ ə /) (Delphinapterus leucas) is an Arctic and sub-Arctic is one of two members of the family Monodontidae, along with the narwhal, and the only member of the genus is also known as the white whale, as it is the only cetacean of this colour; the sea canary, due to its high-pitched calls; and the melonhead, though that more.

The cDNA (DNA complementary to RNA) of the p53 gene of the beluga whale (Delphinapterus leucas) was sequenced by the method of 5′- and 3′-rapid amplification of cDNA ends (RACE) with the cDNA made for the RNA obtained from fresh peripheral blood leukocytes isolated from two s for the RACE method were synthesized based on the sequence of the DNA of beluga whale.

Aromatic DNA adduct levels in liver tissues of beluga whales (Delphinapterus leucas) from two sites in the Canadian Arctic and from the St Lawrence estuary (N.W. Atlantic Ocean) were determined using the highly sensitive 32 P-postlabelling able levels of aromatic DNA adducts (16– nmole adducts/mole total nucleotides) were found in all animals tested.

The ocean is an increasingly industrialized space. Shipping, fishing, and recreational vessels, oil and gas exploration and other human activities all increase noise levels in the ocean and make it more difficult for marine mammals to hear and potentially diminish their range of.

final-scale relatedness patterns of the St. Lawrence River beluga population, and in particular to establish if the population follows a matrilineal social structure, similar to other cetaceans such as sperm whales and orcas.

These queries are addressed by genotyping DNA of beluga whales in the St. Lawrence at 22 microsatellite loci. Frost and SuydamAlaska Beluga Whale Committee (ABWC) unpublished).

Figure 1. Map of the Bering-Chuckhi-Beaufort sea region showing summer distribution of all beluga stocks in the region and the winter distribution of the eastern Bering Sea stock. The ABWC began flying aerial surveys for beluga whales in the EBS in markers (called microsatellites) shows that males move more among stocks than females, but it is not enough to completely mix the DNA.

This means, in general, that the same beluga whales return to the same coastal areas year after year, generation after generation. Thus, each summering group should be managed as a separate stock.

Culture and Genetic Evolution in Whales. Science, a. O'Corry-Crowe GM, Suydam RS, Rosenberg A et al. () Phylogeography, population structure and dispersal patterns of the beluga whale Delphinapterus leucas in the western Nearctic revealed by mitochondrial DNA.

Description Use of DNA microsatellites in beluga whale (Delphinapterus leucas) population genetics EPUB

The short answer is that beluga whales are extremely intelligent, calm in difficult situations and easily trainable, said Pierre Béland, a research scientist in .Whaling is the hunting of whales for their usable products such as meat and blubber, which can be turned into a type of oil which became increasingly important in the Industrial was practiced as an organized industry as early as AD.

By the 16th century, it had risen to be the principal industry in the coastal regions of Spain and France. The beluga whale, also known as the white whale, beluga, melonhead, or sea canary, is a mid-sized cetacean of Arctic and sub-Arctic waters.

This species is .